HomePh.D. Study at IOCBArchive - 2011Organic chemistry

Organic chemistry

Tutor Doc. Ing. Michal Hocek, DSc.
Team Michal Hocek
Topic Construction of functionalized nucleic acids bearing selected reactive functional groups for bioconjugation

Polymerase synthesis of base-modified oligonucleotides and nucleic acids (DNA and RNA) from base-functionalized nucleoside triphosphates (NTPs) will be studied. The starting modified NTPs will be prepared by aqueous cross-coupling reactions. The base-modifications will focus on chemically reactive groups for bioconjugation (i.e. cross-linking with proteins). Further post-synthetic chemical modifications of the functionalized nucleic acids will be also studied. Potential applications will include bioanalysis, diagnostics and chemical biology.

Leading references:
1. Raindlová, V.; Pohl, R.; Šanda, M.; Hocek, M. "Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines" Angew. Chem. Int. Ed. 2010, 49, 1064-1066.
2. Macíčková-Cahová, H.; Hocek, M. " Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases" Nucleic Acids Res. 2009, 37, 7612-7622.
3. Cahová, H.; Havran, L.; Brázdilová, P.; Pivoňková, H.; Pohl, R.; Fojta, M.; Hocek, M. "Aminophenyl- and Nitrophenyl-Labeled Nucleoside Triphosphates. Synthesis, Enzymatic Incorporation and Electrochemical Detection" Angew. Chem. Int. Ed. 2008, 47, 2059-2062.