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Organic Chemistry

Tutor Prof. Ing. Michal Hocek, CSc. DSc.

Michal Hocek

Topic Modifications of DNA by reactive groups for bioconjugations and cross-links

DNA bearing reactive functional groups in major groove will be prepared by polymerase incorporations of modified nucleoside triphosphates. Bioorthogonal reactions of these groups will be studied, e.g. cross-linking with proteins, attachment of other useful functional groups and applications in chemical biology and diagnostics.

Leading references:
1. Raindlová, V.; Pohl, R.; Šanda, M.; Hocek, M. "Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines" Angew. Chem. Int. Ed. 2010, 49, 1064-1066.
2. Dadová, J.; Orság, P.; Pohl, R.; Brázdová, M.; Fojta, M.; Hocek, M. "Vinylsulfonamide and acrylamide modification of DNA for cross-linking with proteins" Angew. Chem. Int. Ed. 2013, 52, 10515-10518.
3. Dadová, J.; Vrábel, M.; Adámik, M.; Brázdová, M.; Pohl, R.; Fojta, M.; Hocek, M. "Azidopropyl-vinylsulfonamide as a new bifunctional click reagent for bioorthogonal conjugations. Application for DNA-protein crosslinking" Chem. Eur. J. 2015, 21, 16091-16102.
4. Olszewska, A.; Pohl, R.; Brázdová, M.; Fojta, M.; Hocek, M. "Chloroacetamide-linked nucleotides and DNA for cross-linking with peptides and proteins" Bioconjug. Chem. 2016, 27, 2089–2094.