HomePhD Study at IOCBTopics 2018Organic Chemistry

Organic Chemistry

Tutor Prof. Ing. Michal Hocek, CSc. DSc.
Team

Michal Hocek

Topic Bioorthogonal reactions on DNA for regulation of transcription
Faculty FCHT / FPBT VŠCHT, PřF UK
Abstract

DNA bearing reactive functional groups in major or minor groove will be prepared by polymerase incorporations of modified nucleoside triphosphates. Bioorthogonal reactions of these groups will be studied, e.g. reactions changing the size and bulkiness of the substituents for switching or triggering of interactions of DNA with proteins or transcription.

Leading references:
1. Macíčková-Cahová, H.; Hocek, M. " Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases" Nucleic Acids Res. 2009, 37, 7612-7622.
2. Kielkowski, P.; Macíčková-Cahová, H.; Pohl, R.; Hocek, M. "Transient and switchable (triethylsilyl)ethynyl protection of DNA against cleavage by restriction endonucleases" Angew. Chem. Int. Ed. 2011, 50, 8727-8730.
3. Raindlová, V.; Janoušková, M.; Slavíčková, M.; Perlíková, P.; Boháčová, S.; Milisavljevic, N.; Šanderová, H.; Benda, M.; Barvík, I.; Krasný, L.; Hocek, M. "Influence of major-groove chemical modifications of DNA on transcription by bacterial RNA polymerases" Nucleic Acids Res. 2016, 44, 3000-3012.
4. Matyašovský, J.; Perlíková, P.; Malnuit, V.; Pohl, R.; Hocek, M. "2-Substituted dATP derivatives as building blocks for polymerase synthesis of DNA modified in minor groove" Angew. Chem. Int. Ed. 2016, 55, 15856 - 15859.
5. Janoušková, M.; Vaníková, Z.; Nici, F.; Boháčová, S.; Vítovská, D.; Šanderová, H.; Hocek, M.; Krásný, L. "5-(Hydroxymethyl)uracil and -cytosine as potential epigenetic marks enhancing or inhibiting transcription with bacterial RNA polymerase" Chem. Commun. 2017, 53, 13253-13255.