HomePh.D. Study at IOCBArchive - 2018Biochemistry and Molecular Biology

Biochemistry and Molecular Biology

Tutor Mgr. Klára Grantz Šašková, Ph.D.
Team Jan Konvalinka
Topic Role of DDI1 protein in DNA repair and transcription regulation
Faculty PřF UK, LF UK

Preservation of DNA integrity throughout the lifetime of each living organism is critical. Many DNA repair processes evolved in both prokaryotic and eukaryotic organisms, employing highly conserved proteins. Defects in the repair pathways underlie numerous human genetic diseases such as developmental syndromes, and further predispose to neurological disorders and cancer. DNA damage-inducible protein 1 (DDI1) is one of the recently discovered guardians of the genome and is also involved in activating transcription factors Nfr1 and Nrf3. The aim of the thesis will be to investigate the function of DDI1 in DNA repair and transcription regulation on molecular level using genetic tools, state-of-the-art mass spectrometry and structural analysis.

Leading references
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2. Trempe et al., Sci Rep 2016, 6, 33671
3. Koizumi et al., eLife 2016, 5, e18357
4. Kottemann et al., Mol Cell 2017 (69), 1, 24-35.e5