HomePh.D. Study at IOCBTopics 2019Biochemistry and Molecular Biology

Biochemistry and Molecular Biology

Tutor Ing. Hana Macickova Cahova, Ph.D.
Team Cahová Group
Topic Role, biogenesis and biodegradation of new RNA caps in prokaryotes
Faculty PřF UK
Abstract

Recent discovery of RNA caps such as NAD and CoA leads to reassessment of RNA structure in all types of cells. In our search for new RNA modifications, we have discovered new 5‘ caps. The task of the thesis is to reveal the role, biogenesis and biodegradation of these new caps. The second task is to develop technique that will allow for identification of particular capped RNA.

References:
Chen, Y. et al. LC/MS analysis of cellular RNA reveals NAD-linked RNA. Nat. Chem. Biol. 5, 879 (2009).
Kowtoniuk, W. et al. A chemical screen for biological small molecule–RNA conjugates reveals CoA-linked RNA. Proc. Natl. Acad. Sci. 106, 7768 (2009).
Cahová, H. et al. NAD captureSeq indicates NAD as a bacterial cap for a subset of regulatory RNAs. Nature, 519, 374 (2015).