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Organic Chemistry

Tutor Doc. Ing. Michal Hocek, Ph.D. DSc.
Team Michal Hocek
Topic Synthesis of modified nucleoside triphosphates and enzymatic construction of functionalized nucleic acids for the study of their interactions with proteins

Novel modified nucleoside triphosphates will be prepared and their polymerase catalyzed incorporations to oligonucleotides and DNA will be studied. The base-modifications will focus on molecular probes (redox-active or fluorescent groups) and chemically reactive groups for bioconjugation (i.e. cross-linking with proteins). Interactions of these modified DNAs with selected DNA-binding proteins (e.g. restriction endonucleases, DNA methyl-transferases etc.) will be studied.

Leading references:
1. Raindlová, V.; Pohl, R.; Šanda, M.; Hocek, M. "Direct polymerase synthesis of reactive aldehyde-functionalized DNA and its conjugation and staining with hydrazines" Angew. Chem. Int. Ed. 2010, 49, 1064-1066.
2. Macíčková-Cahová, H.; Hocek, M. "Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases" Nucleic Acids Res. 2009, 37, 7612-7622.
3. Cahová, H.; Havran, L.; Brázdilová, P.; Pivoňková, H.; Pohl, R.; Fojta, M.; Hocek, M. "Aminophenyl- and Nitrophenyl-Labeled Nucleoside Triphosphates. Synthesis, Enzymatic Incorporation and Electrochemical Detection" Angew. Chem. Int. Ed. 2008, 47, 2059-2062.
4. Kielkowski, P.; Macíčková-Cahová, H.; Pohl, R.; Hocek, M. "Transient and switchable (triethylsilyl)ethynyl protection of DNA against cleavage by restriction endonucleases" Angew. Chem. Int. Ed. 2011, 50, 8727-8730.