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Organic Chemistry

Tutor Doc. Ing. Michal Hocek, CSc. DSc.

Michal Hocek

Topic Synthesis of fluorescently-labelled nucleoside triphosphates and enzymatic construction of labelled nucleic acids for the study of their interactions with proteins

Novel modified nucleoside triphosphates will be prepared and their polymerase catalyzed incorporations to oligonucleotides and DNA will be studied. The base-modifications will focus on environment-selective fluorescent groups (solvatochromic fluorophores, molecular rotors). Interactions of these modified DNAs with selected DNA-binding proteins (e.g. restriction endonucleases, DNA methyl-transferases, transcription factors etc.) will be studied.

Leading references:
1. Riedl, J.; Pohl, R.; Rulíšek, L.; Hocek, M. "Synthesis and Photophysical Properties of Biaryl-Substituted Nucleos(t)ides. Polymerase Synthesis of DNA Probes Bearing Solvatochromic and pH Sensitive Dual Fluorescent and 19F NMR Labels" J. Org. Chem. 2012, 77, 1026-1044.
2. Riedl, J.; Pohl, R.; Ernsting, N. P.; Orsag, P.; Fojta, M.; Hocek M. "Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA-protein interactions" Chem. Sci. 2012, 3, 2797-2806.
3. Riedl, J.; Ménová, P.; Pohl, R.; Orság, P.; Fojta, M.; Hocek, M. "GFP-like fluorophores as DNA labels for studying DNA-protein interactions" J. Org. Chem. 2012, 77, 8287-8293.