HomePh.D. Study at IOCBArchive - 2016Physical Chemistry

Physical Chemistry

Tutor RNDr. Václav Kašička, CSc.
Team Václav Kašička
Topic Investigation of (bio)molecular interactions by capillary electromigration methods
Faculty PřF UK
Abstract

The thesis will be focused on the development of new high-performance electromigration methods, capillary affinity electrophoresis and open-tubular capillary electrochromatography, and on their application to the investigation of the non-covalent interactions of biologically active peptides, proteins, nucleosides, nucleotides and other (bio)molecules with low- and high-molecular-mass ligands or receptors. New procedures will be developed for quantitative characterization of both weak and strong complexes of (bio)molecules in a free solution and on the interface of the liquid and solid phases. Interactions of biomolecules, e.g. peptide hormones or drugs with receptors, enzymes with substrates and inhibitors, and ionophores and functional organic molecules with small ions in a free solution will be investigated by different modes of capillary affinity electrophoresis. Interactions of biomolecules on the interface of solid and liquid phases will be studied by open tubular capillary electrochromatography with ligands or receptors immobilized on the inner wall of the fused silica capillary. Strength of the interactions of (bio)molecules will be quantified by the stability constants of their complexes.