Tutor Ing. Marie Zarevúcka, CSc.
Team Irena Valterová
Topic Enzymic reactions in supercritical fluids

Enzymic reactions in supercritical carbon dioxide combine the advantage of biocatalysis (substrate specifity under mild reaction conditions) and supercritical fluids (high mass-transfer rate, easy separation of reaction products from the solvent, environmental benefits).

Ph.D. student will study the following tasks:
i) isolation and preparation of enzymes from Geotrichum candidum
ii) testing the enzyme activity and stability in supercritical carbon dioxide
iii) investigating the enzyme reaction in a continuous-flow reactor and the conversion of the reactions and specificity of enzymes
iv) understanding the kinetic, thermodynamic, and transport phenomena and their influence on the enzyme-catalyzed reactions.