Jaroslav Šebestík, PhD.
+420 220 183 445
Selected papers
Photochemical synthesis of pink silver and its use for monitoring peptide nitration via surface enhanced Raman spectroscopy (SERS)
Amino Acids 54: 1261–1274 (2022)
Oxidative stress may cause extended tyrosine posttranslational modifications of peptides and proteins. The 3-nitro-L-tyrosine (Nit), which is typically formed, affects protein behavior during neurodegenerative processes, such as Alzheimer’s and Parkinson’s diseases. Such metabolic products may be conveniently detected at very low concentrations by surface enhanced Raman spectroscopy (SERS). Previously, we have explored the SERS detection of the Nit NO2 bending vibrational bands in a presence of hydrogen chloride (Niederhafner et al., Amino Acids 53:517–532, 2021, ibid). In this article, we describe performance of a new SERS substrate, “pink silver”, synthesized photochemically. It provides SERS even without the HCl induction, and the acid further decreases the detection limit about 9 times. Strong SERS bands were observed in the asymmetric (1550–1475 cm−1) and symmetric (1360–1290 cm−1) NO stretching in the NO2 group. The bending vibration was relatively weak, but appeared stronger…
Monitoring peptide tyrosine nitration by spectroscopic methods
Amino Acids 2021: 517–532
Observation of Paramagnetic Raman Optical Activity of Nitrogen Dioxide
Angewandte Chemie International Edition 53 (35): 9236-9239 (2014)
Latest publications
Acidobasic equilibria of inubosin derivatives studied by UV–Vis spectroscopy
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 334: 125950 (2025)
Mueller matrix polarimetry reveals chiroptical properties of metal chelates in solutions
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 339: 126279 (2025)
Conformations and hydration of halopropionic acids studied by molecular dynamics and Raman optical activity
309: 123852 (2024)
Discovery of a Druggable, Cryptic Pocket in SARS-CoV-2 nsp16 Using Allosteric Inhibitors
ACS Infectious Diseases 9 (10): 1918–1931 (2023)