Orbitrap IQ-X Tribrid

UltrafleXtreme MALDI-TOF/TOF mass spectrometer (Bruker Daltonics, Germany) is the+ instrument combining true 1 kHz smartbeam II laser technology speed in both TOF and TOF/TOF modes with ultra-high performance and extreme flexibility. The instrument was installed in 2011. It is used for rapid analysis of biomolecules and synthetic compounds. It is also used to study the distribution of chemical species in tissue sections (MALDI molecular imaging).

The SCIEX QTRAP 6500+ system combines triple quadrupole mass spectrometry with linear ion trap (LIT). The system consists of serially connected three quadrupoles with a potential of using the last one as LIT for improved MS scans, such as Enhanced MS (EMS), Enhanced Multi-Charge (EMC), Enhanced Product Ion (EPI), Enhanced Resolution (ER) and MS/MS/MS (MS3). The QTRAP 6500+ system connects with ExionLC UHPLC system through atmospheric pressure ion sources – ESI or APCI. The QTRAP 6500+ system also offers connection of an optional module – differential mobility spectrometry (DMS, SelexION technology) thus allowing separation of isobaric species based on their collision cross section.

At the research-service team of Mass Spectrometry, the QTRAP system is used for quantification in general and targeted metabolomics studies. Such setup enables separation and targeted quantification of small molecules, e.g., neurotransmitters, nucleosides/nucleotides, phytoprostanes, especially when using multiple reaction monitoring mode (MRM).

Orbitrap Fusion Lumos Tribrid mass spectrometr (Thermo Scientific) is mass spectrometer combines quadrupole, ion traps and orbitrap mass analyzer. According the mentioned combination, Thermo Scientific provider called the instrument tribrid. This system incorporates the brightest ion source, a segmented quadrupole mass filter with improved selectivity and ion transmission, Advanced Vacuum Technology for improved ion transmission to the Orbitrap mass analyzer. The instrument provide the possibility of well-established ionizations as ESI, nanoESI, APCI and APPI and fragmentation CID, HCD and ETD. The instrument provides 1,000,000 FWHM ultra-high resolution for improved structural elucidation and quantitation of isobaric compounds. The instrument is connected to two HPLC systems; UltiMate 3000 RSLCnano and UltiMate 3000 RS HPLC systems (Thermo Scientific) for HPLC in nanoliters and microliters flow rates, respectively. The instrument was purchased from the OP RDE project "Chemical biology for drugging undruggable targets (ChemBioDrug)" in 2018 and is used for the solution of the task connected with the project.

The SYNAPT G2 (Waters) is a tandem hybrid mass spectrometer that utilizes a quadrupole and a time-of-flight (TOF) mass analyzer, and is further equipped with a separation cell enabling ion mobility based on a traveling wave. This instrument is primarily used for service-based analysis of the intact mass of proteins and nucleic acids, analysis of covalent modifications, and covalent protein–protein and protein–ligand complexes.

LTQ Orbitrap XL (Thermo Scientific) ) is a hybrid FT mass spectrometer combines a linear ion trap MS and the Orbitrap mass analyzer. Ions generated by API (APCI, ESI, nanoESI) are collected in the LTQ XL followed by axial ejection to the C-shaped storage trap, which is used to store and collisionally cool ions before injection into the orbitral trap. Excellent mass accuracy and its stability are the key features of this instrument. LTQ Orbitrap XL can be coupled to UHPLC system for LC/MS applications. In our lab, the instrument is mainly used for determination of elemental composition of organic compounds and modified biomolecules based on accurate mass measurement. The instrument was installed in January 2008.

LCQ (Thermo Scientific) ion-trap mass spectrometers is used for analytical studies. In one instrument, a modified inlet system allows the introduction of various samples in the ionization as well as the reaction regions. LCQ Classic is coupled to capillary electrophoresis.

The SCIEX Echo MS system consists of triple quadrupole mass spectrometer (SCIEX Triple Quad 6500+) and an Echo MS module, which is an analyte sampler based on acoustic wave system called Acoustic Droplet Ejection combined with Open Port Interface design. The ejection of droplets takes place from a 384 well plate. Mass spectrometer and Echo MS module are connected via capillary that also serves as an ESI source.

The Echo MS system is used at the research-service group biochemical pharmacology for targeted quantification in ADME assays and enzyme assays, where samples contain diverse and complex matrices. Multiple reaction monitoring (MRM) mode is used for the targeted quantification. The system is especially useful in high-throughput quantitative analysis, since the speed of analysis can be as fast as 1 sample per second.

Thw SELECT SERIES Cyclic IMS mass spectrometer (Waters Corporation, USA) combines a quadrupole analyzer and a time-of-flight (TOF) mass analyzer with a cyclic ion mobility separation cell. The instrument is equipped with ESI and nano-ESI sources. Cyclic ion mobility enables the separation of isomers and conformers based on their collision cross-section. The resolution of ion mobility separation increases with the number of cycles within the mobility cell, allowing the separation to be adapted to the sample’s complexity. Additionally, the instrument supports electron capture dissociation (ECD) and surface-induced dissociation (SID) techniques.

The instrument is used for service measurements of hydrogen-deuterium exchange (HDX-MS) and for native analysis of intact proteins and nucleic acids, including their non-covalent protein–protein and protein–ligand complexes.

The SELECT SERIES Cyclic IMS mass spectrometer was installed in December 2021.

The Orbitrap ID-X™ Tribrid™ mass spectrometer (Thermo Scientific) combines three mass analyzers: a quadrupole, an Orbitrap, and a linear ion trap. The quadrupole allows for precursor ion selection and fragmentation. The Orbitrap provides high-resolution mass measurements. The linear ion trap enables MSⁿ (tandem mass spectrometry) experiments. This configuration significantly expands the possibilities for the analysis of unknown compounds, and it is an invaluable technology in the fields of untargeted metabolomics and lipidomics, as well as in the study of oligonucleotides and natural compounds. The instrument was installed in the summer of 2020.