LEAP PAL robot combined with HDX system

  • Other Instruments
Image of LEAP PAL robot combined with HDX system

The system for hydrogen-deuterium exchange (HDX) studies is equipped with the latest generation of HDX-MS instrumentation from Waters Corporation composed of a Synapt G2 mass spectrometer with electrospray ionization and CID fragmentation technique, an ACQUITY UPLC M-Class system with HDX manager, and LEAP-Pal robot for automated sample preparation and handling providing high data reproducibility. The option to apply the protease columns with different substrate specificity from Affipro company for online digestion of proteins solves the widespread problem with reaching the sufficient protein sequence coverage. It provides sufficient sequence coverage of studied protein crucial for deuterium uptake mapping and thus it facilitates the data interpretation as itself.

In our lab, system for HDX is used for the study of protein-protein or protein-ligand interactions.

LEAP PAL robot combined with HDX system was installed in December 2021.