In cooperation with scientists from the University of York and the Institute of Microbiology of the CAS, a team led by Irena Selicharová from Jiří Jiráček Group at IOCB Prague has uncovered protein-protein interactions concerning human insulin-like growth factor (IGF-1) that weren’t determined prior using X-ray crystallography.
The researchers used a different technique, combining chemical cross-linking using a bifunctional linker (Sulfo-SDA) and mass spectrometry.
These findings show the viability of such an approach as a complement to crystallographic and other structure-based methods when studying such interactions and protein interaction interfaces.
Read the paper:
- Pompach, P.; Viola, C. M.; Radosavljević, J.; Lin, J.; Jiráček, J.; Brzozowski, A. M.; Selicharová, I. Cross-Linking/Mass Spectrometry Uncovers Details of Insulin-Like Growth Factor Interaction With Insect Insulin Binding Protein Imp-L2. Frontiers in Endocrinology 2019, 10. https://doi.org/10.3389/fendo.2019.00695
Read next...
