Novel squaramate-linked nucleotide was designed, synthesized, and used as a building block for enzymatic synthesis of modified DNA which then undergoes selective cross-linking with Lys-containing peptides and proteins under physiological conditions.
The new reactive group and cross-linking reaction extends the portfolio of methods for synthesis of DNA-protein conjugates and has the potential for applications in identification and study of DNA-binding proteins.
Read the paper:
- Ivancová, I.; Pohl, R.; Hubálek, M.; Hocek, M. Squaramate-Modified Nucleotides and DNA for Specific Cross-Linking with Lysine-Containing Peptides and Proteins. Angewandte Chemie International Edition 2019, 58, 13345-13348. https://doi.org/10.1002/anie.201906737
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