Profile

EDUCATION
1998-2003

M.Sc. in Biochemistry at the Faculty of Science, Charles University, Prague, Czech Republic

Diploma thesis : „The viral resistance in HIV positive patiens threated with inhibitors of HIV protease“

2006-2010

Combined postgradual study at the Faculty of Science, Charles University and the Institute of Organic Chemistry and Biochemistry, v.v.i., Academy of Sciences of the Czech Republic, Prague

Dissertation thesis : „Overcoming drug resistance: The discovery, design and characterization of new nonpeptidic inhibitors of HIV-1 protease“.

RESEARCH AND ACADEMIC POSITIONS
since 2010

Senior scientist at the Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague (group of Jan Konvalinka, Ph.D.)

since 2018

Ph.D. supervisor at the First Faculty of Medicine, Charles University in Prague

AWARDS
2014

The Otto Wichterle Award for selected, exceptionally outstanding, promising young scientists at the Academy of Sciences of the Czech Republic for their remarkable contributions to the advancement of scientific knowledge in a given area of science

2011

The Josef Hlávka Award for the best students and graduates of Prague public universities, Brno Technical University and young talented academics of the Academy of Sciences of the Czech Republic

2008

Chairmen´s award for the best poster „Kinetic, Structural and Thermodynamic Characterization of HIV Protease with Insertion in the Flap Region Isolated from and HIV-Positive Patient“ at the conference Applications of Biocalorimetry (abc6), Heidelberg, Germany

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MAIN RESEARCH INTERESTS

Rational design and characterization of inhibitors targeting influenza polymerase
Hejdánek et al. Antivir Res 2021; Gregor et al. Molecules 2021; Zima et al. Eur J Med Chem 2020

Rational design and characterization of inhibitors targeting influenza neuraminidase
Zima et al. Bioorg Med Chem 2019; Pokorná et al. Viruses 2018; Berenguer Albiñana et al. Eur J Med Chem 2016

Ultrasensitive microcalorimetry in the study of biomolecular interactions and protein stability
Vavřina et al. Biochemistry 2021; Rulíšek et al. Angew Chem Int Ed 2021; Began et al. EMBO J 2020; Heyda et al. J Phys Chem B 2011; Vaněk et al. J Med Chem 2009; Řezáčová et al. Mol Microbiol 2008; Král et al. Proteins 2008; Kožíšek et al. Eur J Biochem 2008; Vondrášek et al. Chem Eur J 2007

Protein-protein and protein-ligand interaction techniques (HTS, AlphaScreen, SPR, ITC, DSC)
Kožíšek et al. Biochem J 2018; Trempe et al. Sci Rep 2016; Sivá et al. Sci Rep 2016; Németh et al. J Inorg Biochem 2015; Rozbeský et al. Int J Mol Sci 2014; Németh et al. Protein Sci 2014; Németh et al. J Biol Inorg Chem 2014; Motáčková et al. Biomol NMR Assign 2009

Viral resistance development in HIV positive patiens treated with inhibitors of HIV protease
Kožíšek et al. FEBS J 2014; Grantz Šašková et al. J Virol 2014; Schimer et al. J Med Chem 2012; Kožíšek et al. Antimicrob Agents Chemother 2012; Grantz Šašková et al. J Virol 2009; Grantz Šašková et al. Protein Sci 2008; Bartoňová et al. Antivir Res 2008; Kožíšek et al. J Mol Biol 2007

Rational design and characterization of HIV protease inhibitors based on metallacarboranes
Řezáčová et al. J Med Chem 2009; Kožíšek et al. J Med Chem 2008; Kubát et al. J Phys Chem B 2007; Cígler and Kožíšek et al. Proc Natl Acad Sci USA 2005

Research of the insertions in HIV protease
Kožíšek et al. J Virol 2008

HIV capsid and viral assembly
Machara et al. J Med Chem 2016; Kožíšek et al. Bioorg Med Chem Lett 2016; Doležal et al. J Biol Chem 2016

Latest publications

Synthesis and In Vitro Evaluation of C-7 and C-8 Luteolin Derivatives as Influenza Endonuclease Inhibitors
International Journal of Molecular Sciences 22 (14): 7735 (2021)
Ligand Strain and Its Conformational Complexity Is a Major Factor in the Binding of Cyclic Dinucleotides to STING Protein
Angewandte Chemie International Edition 60 (18): 10172-10178 (2021)
Protein–Ligand Interactions in the STING Binding Site Probed by Rationally Designed Single-Point Mutations: Experiment and Theory
Biochemistry 60 (8): 607–620 (2021)
Structural and Thermodynamic Analysis of the Resistance Development to Pimodivir (VX-787), the Clinical Inhibitor of Cap Binding to PB2 Subunit of Influenza A Polymerase
Molecules 26 (4): 1007 (2021)