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Ústav organické chemie a biochemie
AV ČR

Vědci z ÚOCHB k epidemii nového koronaviru v médiích
Structural analysis of the SARS-CoV-2 methyltransferase complex involved in RNA cap creation bound to sinefungin
Structural analysis of the SARS-CoV-2 methyltransferase complex involved in RNA cap creation bound to sinefungin
Nature Communications 11 : 3717 (2020).
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the COVID-19 pandemic. 2′-O-RNA methyltransferase (MTase) is one of the enzymes of this virus that is a potential target for antiviral therapy as it is crucial for RNA cap formation; an essential process for viral RNA stability. This MTase function is associated with the nsp16 protein, which requires a cofactor, nsp10, for its proper activity. Here we show the crystal structure of the nsp10-nsp16 complex bound to the pan-MTase inhibitor sinefungin in the active site. Our structural comparisons reveal low conservation of the MTase catalytic site between Zika and SARS-CoV-2 viruses, but high conservation of the MTase active site between SARS-CoV-2 and SARS-CoV viruses; these data suggest that the preparation of MTase inhibitors targeting several coronaviruses - but not flaviviruses - should be feasible. Together, our data add to important information for structure-based drug discovery.
Toll-like receptor dual-acting agonists are potent inducers of PBMC-produced cytokines that inhibit hepatitis B virus production in primary human hepatocytes
V. Janovec
V. Janovec
J. Hodek K. Clarová T. Hofman
T. Hofman
P. Dostalík
J. Froněk
J. Chlupáč
L. Chaperot
S. Durand
T. F. Baumert
I. Pichová B. Lubyová I. Hirsch J. Weber
Scientific Reports 10 : 12767 (2020).
Mass spectrometry imaging of free-floating brain sections detects pathological lipid distribution in a mouse model of Alzheimer's-like pathology
Analyst 145 (13): 4595-4605 (2020).
Morphing radial molecular property functions of hydroxyl
L. D. Augustovičová
V. Špirko
Journal of Quantitative Spectroscopy and Radiative Transfer 254 : Early View (2020).
Relativistic Heavy-Neighbor-Atom Effects on NMR Shifts: Concepts and Trends Across the Periodic Table
J. Vícha
J. Vı́cha
J. Novotný
S. Komorovsky
M. Straka
M. Kaupp
R. Marek
Chemical Reviews 2020 : Early View (2020).

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