Zvaná přednáška – Nenad Ban

20. června 2022
ÚOCHB
Zvaná přednáška – Nenad Ban
Přednášející

Prof. Nenad Ban (Institute of Molecular Biology and Biophysics, ETH Zurich, Švýcarsko)

Téma

Structural Basis of Protein Synthesis in Eukaryotes: from Basic Principles to Translation in Coronavirus Infected Cells

Datum a čas

20. června 2022, 10:00

Místo

Přednáškový sál ÚOCHB

Abstrakt

We are investigating bacterial and eukaryotic ribosomes and their functional complexes to obtain insights into the process of protein synthesis. Building on our studies aimed at revealing the structures of eukaryotic cytosolic and mitochondrial ribosomes, we are now investigating eukaryotic translation initiation, targeting of proteins to membranes, regulation of protein synthesis, and how viruses reprogram host translation. Previously, we studied how Hepatitis C virus genomic RNA can bind mammalian ribosomes to achieve translation of viral mRNAs in the absence of some canonical cellular translation initiation factors (Quade et al. 2015). With our recent research activities we contributed to the understanding of how SARS-CoV-2, the virus that is responsible for the COVID-19 pandemic, shuts off host translation to prevent cellular defence mechanisms against the virus (Schubert et al. 2020). Furthermore, using a combination of cryo-electron microsocpy and biochemical assays we also investigated the mechanism of programmed ribosomal frameshifting, one of the key events during translation of the SARS-CoV-2 RNA genome that leads to synthesis of the viral RNA-dependent RNA polymerase and downstream viral proteins (Bhat et al. 2020). 

Zdroje

  • Quade N, Boehringer D, Leibundgut M, van den Heuvel J, Ban N. Cryo-EM structure of Hepatitis C virus IRES bound to the human ribosome at 3.9-Å resolution. Nat. Commun. 2015, 6, 7646.
  • Schubert K, Karousis ED, Jomaa A, Scaiola A, Echeverria B, Gurzeler LA, Leibundgut M, Thiel V, Mühlemann O, Ban N. (2020) SARS-CoV-2 Nsp1 binds the ribosomal mRNA channel to inhibit translation. Nat. Struct. Mol. Biol. 2020, 27, 959-966.
  • Bhatt PR, Scaiola A, Loughran G, Leibundgut M, Kratzel A, Meurs R, Dreos R, O'Connor KM, McMillan A, Bode JW, Thiel V, Gatfield D, Atkins JF, Ban N. (2021) Structural basis of ribosomal frameshifting during translation of the SARS-CoV-2 RNA genome. Science 2021, 372 (6548), 1306-1313. 

Fotogalerie

Zleva: Dr. Irena Stará, organizátorka Zvaných přednášek ÚOCHB; Prof. Jan Konvalinka, ředitel ÚOCHB; Prof. Nenad Ban, ETH Zurich; doc. Pavlína Maloy Řezáčová, zástupkyně ředitele ÚOCHB pro výzkum. Foto: Michal Hoskovec / ÚOCHB Foto: Michal Hoskovec / ÚOCHB Foto: Michal Hoskovec / ÚOCHB Foto: Michal Hoskovec / ÚOCHB Foto: Michal Hoskovec / ÚOCHB Foto: Michal Hoskovec / ÚOCHB
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