UltrafleXtreme™ MALDI-TOF/TOF mass spectrometer (Bruker Daltonics, Germany) is the only instrument combining true 1 kHz smartbeam II laser technology speed in both TOF and TOF/TOF modes with ultra-high performance and extreme flexibility. The instrument was installed in 2011.
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The SCIEX TripleTOF 5600 is the next generation in Quadrupole TOF technology. Starting with the best-in-class Triple Quad 5500 System, the new AcceleratorTOF Analyzer was developed to add high-resolution at the speed and sensitivity needed to give equivalent CV's and limits of quantitation (LOQ) to high-end triple quadrupoles. The mass spectrometers uniquely integrates comprehensive qualitative exploration, rapid profiling, and high-resolution quantitation workflows on a single platform. It combines the highest sensitivity detection, high-resolution with 100 Hz acquisition speed, and stable ~1 ppm mass accuracy over days of acquisition. The spectrometer is connected with Dionex ULTIMATE 3000 HPLC system. The instrument was installed in summer 2011.
The SCIEX QTRAP 6500+ system combines triple quadrupole mass spectrometry with linear ion trap (LIT). The system consists of serially connected three quadrupoles with a potential of using the last one as LIT for improved MS scans, such as Enhanced MS (EMS), Enhanced Multi-Charge (EMC), Enhanced Product Ion (EPI), Enhanced Resolution (ER) and MS/MS/MS (MS3). The QTRAP 6500+ system connects either with ExionLC UHPLC or with the NanoLC 425 system through atmospheric pressure ion sources – ESI or APCI. The QTRAP 6500+ system also offers connection of an optional module – differential mobility spectrometry (DMS, SelexION technology) thus allowing separation of isobaric species based on their collision cross section.
At the research-service team of Mass Spectrometry, the QTRAP system is used for targeted metabolomics studies. Such setup enables separation and targeted quantification of small molecules, e.g., neurotransmitters, nucleosides/nucleotides, phytoprostanes, especially when using multiple reaction monitoring mode (MRM).
Orbitrap Fusion Lumos Tribrid mass spectrometr (Thermo Scientific) is mass spectrometer combines quadrupole, ion traps and orbitrap mass analyzer. According the mentioned combination, Thermo Scientific provider called the instrument tribrid. This system incorporates the brightest ion source, a segmented quadrupole mass filter with improved selectivity and ion transmission, Advanced Vacuum Technology for improved ion transmission to the Orbitrap mass analyzer. The instrument provide the possibility of well-established ionizations as ESI, nanoESI, APCI and APPI and fragmentation CID, HCD and ETD. Our high-end instrument include also innovations as UVPD, a new fragmentation technique achieved with a 213 nm UV laser, and provides 1,000,000 FWHM ultra-high resolution for improved structural elucidation and quantitation of isobaric compounds. The instrument is connected to two HPLC systems; UltiMate 3000 RSLCnano and UltiMate 3000 RS HPLC systems (Thermo Scientific) for HPLC in nanoliters and microliters flow rates, respectively. The instrument was purchased from the OP RDE project "Chemical biology for drugging undruggable targets (ChemBioDrug)" in 2018 and is used for the solution of the task connected with the project.
The SYNAPT G2 (Waters) is hybrid quadrupole - time-of-flight (Q-TOF) mass spectrometer equipped with a travelling wave ion-mobility separation cell, which allows to separate gaseous ions not only in terms of mass, but also in terms of their shapes. Q-Tof micro is coupled to Acquity UPLC M-Class and I-Class system (Waters). ESI, nanoESI, APCI and MALDI ion sources are available. Many options allowed to employ the instrument to many scientific project, for example, analysis of dinucleoside polyphosphates, development top-down MALDI in source decay based proteomics, analysis of lipopeptides and mobility separation of G-quadruplexes.
LTQ Orbitrap XL (Thermo Scientific) is a hybrid FT mass spectrometer combines a linear ion trap MS and the Orbitrap mass analyzer. Ions generated by API (APCI, ESI, nanoESI) are collected in the LTQ XL followed by axial ejection to the C-shaped storage trap, which is used to store and collisionally cool ions before injection into the orbitral trap. Excellent mass accuracy and its stability are the key features of this instrument. LTQ Orbitrap XL can be coupled to UHPLC system for LC/MS applications. In our lab, the instrument is mainly used for determination of elemental composition of organic compounds and modified biomolecules based on accurate mass measurement. The instrument was installed in January 2008.
Q-Tof micro (Waters) is a quadrupole orthogonal acceleration time-of-flight tandem mass spectrometer that enables exact mass measurement in both MS and MS/MS modes of analysis. ESI, nano-ESI and APCI ion sources are available. Q-Tof micro is coupled to CapLC capillary HPLC for tryptic digest separations.
LCQ (Thermo Scientific) ion-trap mass spectrometers is used for analytical studies. In one instrument, a modified inlet system allows the introduction of various samples in the ionization as well as the reaction regions. LCQ Classic is coupled to capillary electrophoresis.